Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4020

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Agarose Gel Electrophoresis

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 100 words of the full text of this article appear below.


INTRODUCTION

How to pour, load, and run an agarose gel.


MATERIALS

recipe 6x Gel-loading buffer

Agarose solutions (please see Step 3)

DNA samples

DNA size standards

Samples of DNAs of known size are typically generated by restriction enzyme digestion of a plasmid or bacteriophage DNA of known sequence. Alternatively, they are produced by ligating a monomer DNA fragment of known size into a ladder of polymeric forms.

recipe DNA staining solution

For a discussion of staining agarose gels, please see Detection of DNA in Agarose Gels.

recipe Electrophoresis buffer


METHOD

1. Seal the edges of a clean, dry glass plate (or the open ends of . . . [Full Text of this Article]


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