Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4151

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protocolProtocol

Preparation of Genomic DNA from Yeast Using Glass Beads

David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern

This protocol was adapted from "Yeast DNA Isolations," Techniques and Protocols 3, in Methods in Yeast Genetics, 2005 edition, by David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005).

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes the preparation of genomic DNA from a 5-ml liquid culture of yeast cells.


MATERIALS

Reagents

recipe caution Ammonium acetate, 5M

Appropriate yeast cultures

caution Chloroform:isoamyl alcohol(24:1, v:v)

recipe EDTA-Sark

Ethanol, 95%, cold

Ethanol, 70%

Growth medium, rich or selective (see Step 1)

recipe Yeast lysis buffer II

recipe NaCl, 5 M

recipe caution Phenol , equilibrated with H2O

recipe caution Proteinase . . . [Full Text of this Article]

Equipment


METHOD


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