Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4318

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protocolProtocol

Cosuppression in C. elegans

René F. Ketting, Marcel Tijsterman, and Ronald H.A. Plasterk

This protocol was adapted from "RNAi in C. elegans," contributed by René F. Ketting, Marcel Tijsterman, and Ronald H.A. Plasterk, Chapter 4, in RNAi: A Guide to Gene Silencing (ed. Hannon). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

Cosuppression is a process in Caenorhabditis elegans that closely resembles RNAi. In contrast to RNAi, however, the cosuppression effect in C. elegans does not spread throughout the animal. Cosuppression in C. elegans can be triggered by highly repetitive transgenes that contain gene constructs . . . [Full Text of this Article]


MATERIALS

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METHOD


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