Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4273

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Harlow, E.
Right arrow Articles by Lane, D.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Harlow, E.
Right arrow Articles by Lane, D.
Related Collections
Right arrow Antibodies, general
Right arrow Molecular Biology, general
Right arrow Immunoblotting
Right arrow Proteins and Proteomics, general
Right arrow Blots
Right arrowRelated Protocols
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
Legend icon

protocolProtocol

Stripping Immunoblots for Reprobing or Storage

Ed Harlow and David Lane

This protocol was adapted from "Immunoblotting," Chapter 8, in Using Antibodies by Ed Harlow and David Lane. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1999.

The first 15% of the full text of this article appears below.


INTRODUCTION

The practice of stripping immunoblots and reprobing to detect multiple proteins on the same blot is a good method to examine two antigens under identical conditions. However, this approach should not . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


TROUBLESHOOTING


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related Protocols

Immunoblotting: Antigen Detection Using Chemiluminescence
Ed Harlow and David Lane
Cold Spring Harb Protoc 2006: 4271. [Extract] [Full Text]

Immunoblotting: Antigen Detection Using Chromogenic Methods
Ed Harlow and David Lane
Cold Spring Harb Protoc 2006: 4272. [Extract] [Full Text]