Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4282

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Storage of Purified Antibodies

Ed Harlow and David Lane

This protocol was adapted from "Handling Antibodies," Chapter 4, in Using Antibodies by Ed Harlow and David Lane. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1999.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes the storage of "homemade" purified antibodies. Antibodies from commercial sources are normally supplied with proper storage conditions. For purification protocols, please see Antibody Purification on Protein A or Protein G Columns and Purification of Antibodies on an Antigen Column. Sodium azide to 0.02% (or Merthiolate to . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


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Antibody Purification on Protein A or Protein G Columns
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