Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4288

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Harlow, E.
Right arrow Articles by Lane, D.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Harlow, E.
Right arrow Articles by Lane, D.
Related Collections
Right arrow Antibodies, general
Right arrow Handling Antibodies
Right arrow Handling Antibodies, general
Right arrow Labeling Antibodies
Right arrow Monoclonal Antibodies
Right arrow Immunology, general
Right arrowRelated Protocols
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

protocolProtocol

Labeling Monoclonal Antibodies by Biosynthesis

Ed Harlow and David Lane

This protocol was adapted from "Handling Antibodies," Chapter 4, in Using Antibodies by Ed Harlow and David Lane. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1999.

The first 15% of the full text of this article appears below.


INTRODUCTION

This method for tagging monoclonal antibodies involves growing hybridomas in the presence of radioactive amino acids. This protocol can be particularly useful when conventional labeling techniques cause the antibody to lose activity. The labeled antibodies that result are essentially identical to the unlabeled antibodies.


MATERIALS

Reagents

Hybridoma cells that produce the monoclonal antibody of . . . [Full Text of this Article]

Equipment


METHOD


TROUBLESHOOTING


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related Protocols

Antibody Purification on Protein A or Protein G Columns
Ed Harlow and David Lane
Cold Spring Harb Protoc 2006: 4283. [Extract] [Full Text]

Purification of Antibodies on an Antigen Column
Ed Harlow and David Lane
Cold Spring Harb Protoc 2006: 4284. [Extract] [Full Text]