Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4386

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Nephrectomy

Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer

This protocol was adapted from "Surgical Procedures," Chapter 6, in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes surgical removal of a mouse kidney to provide tissue for analysis.


MATERIALS

Reagents

recipe caution Appropriate anesthetic

Mouse

Equipment

Animal clippers

Clean cage

Dissection scissors

Fiber-optic illuminator (optional)

Forceps, blunt

Forceps, watchmaker’s #5, two pairs

Needle, 26-gauge, 1/2 inch

Needle, curved surgical (e.g., size 10, triangular, pointed)

Suture, surgical silk . . . [Full Text of this Article]


METHOD


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Related Protocol

Intraperitoneal (IP) Injection
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
Cold Spring Harb Protoc 2006: 4352. [Extract] [Full Text]