Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot3914

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Removal of Ethidium Bromide from DNA by Extraction with Organic Solvents

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

Ethidium bromide is removed from DNA by phase extraction with organic solvents.


MATERIALS

cautionn-Butanol, saturated with H2O

DNA sample, purified through CsCl gradient

Use material from either Step 7 of Purification of Closed Circular DNA by Equilibrium Centrifugation in CsCl-Ethidium Bromide Gradients: Continuous Gradientsor Step 5 of Purification of Closed Circular DNA by Equilibrium Centrifugation in CsCl-Ethidium Bromide Gradients: Discontinuous Gradients.

Ethanol

Optional, please see Step 5.

Isoamyl alcohol, saturated with H2O

recipe caution Phenol

caution Phenol:chloroform . . . [Full Text of this Article]


METHOD


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