Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4039

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protocolProtocol

Rapid Isolation of Yeast DNA

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

This method is used to isolate genomic yeast DNA or shuttle plasmids that replicate in both S. cerevisiae and E. coli. The DNA can be used as a template for PCR and for transformation.


MATERIALS

Ethanol

Optional, please see Step 5.

caution Phenol:chloroform (1:1, v/v)

recipe STES buffer

recipe caution Sodium acetate (3 M, pH 5.2)

recipe TE (pH . . . [Full Text of this Article]


METHOD


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