Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4044

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sambrook, J.
Right arrow Articles by Russell, D. W.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Sambrook, J.
Right arrow Articles by Russell, D. W.
Related Collections
Right arrow Molecular Biology, general
Right arrow Genomic DNA
Right arrow Probes, general
Right arrow Radiolabeled Probes
Right arrow Blots
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

protocolProtocol

Southern Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Membranes

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 100 words of the full text of this article appear below.


INTRODUCTION

This protocol describes how to carry out Southern hybridizations at high stringency in phosphate-SDS buffers. Although a wide variety of formats are available, most Southern hybridizations are carried out in heat-sealable bags, roller bottles, or plastic boxes, as described here.


MATERIALS

recipe 0.1x SSC

recipe 0.1x SSC with 0.1% (w/v) SDS

recipe 2x SSC with 0.1% (w/v) SDS

recipe 2x SSC with 0.5% (w/v) SDS

recipe 6x SSC or

recipe 6x SSPE

DNA immobilized on membrane

recipe Phosphate-SDS washing solution 1

recipe Phosphate-SDS washing solution 2

Poly(A) RNA (10 mg/ml) in sterile H2O

Optional, for hybridization buffers. Prepare solution by dissolving poly(A) RNA in sterile H2O and . . . [Full Text of this Article]


METHOD


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
A. E. Davidson, T. E. Gratsch, M. H. Morell, K. S. O'Shea, and C. E. Krull
Use of the Sleeping Beauty Transposon System for Stable Gene Expression in Mouse Embryonic Stem Cells
Cold Spring Harb Protoc, August 1, 2009; 2009(8): pdb.prot5270 - pdb.prot5270.
[Abstract] [Full Text]