Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4292

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Attaching Suspension Cells to Slides Using Chemical Linkers

Ed Harlow and David Lane

This protocol was adapted from "Staining Cells," Chapter 5, in Using Antibodies by Ed Harlow and David Lane. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1999.

The first 15% of the full text of this article appears below.


INTRODUCTION

A simple method for handling suspension cells is to attach them to a solid substrate by chemical linkage before fixation. For chemical linkage, a positively charged polymer (commonly poly-L-lysine or a commercial equivalent such as Vectabond) provides a site for binding of cells, which carry an overall negative charge. . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


TROUBLESHOOTING


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