Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4299

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Immunoblotting: Preparing Cell Lysates

Ed Harlow and David Lane

This protocol was adapted from "Immunoblotting," Chapter 8, in Using Antibodies by Ed Harlow and David Lane. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1999.

The first 15% of the full text of this article appears below.


INTRODUCTION

For immunoblotting experiments, it is often important to compare the total amount of an antigen from many different sources or to learn if a particular source has the antigen under study. In the approach described here, tissue cultures, bacteria, yeast cells, tissues, and other sources of antigens are disrupted directly in an electrophoresis sample . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


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