Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4423

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Assembling Aggregates between Diploid Embryos

Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer

This protocol was adapted from "Production of Chimeras," Chapter 11, in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes a method for assembling aggregates between diploid embryos. If embryos from a heterozygous mutant intercross are aggregated with wild-type embryos, the resulting chimeras can be used for analyzing mutant phenotypes.


MATERIALS

Reagents

Embryos, 2.5-days post coitum (dpc) (see Collecting Two-Cell- to Compacted Morula-Stage Embryos)

recipe KSOM-AA medium or other embryo culture medium

Equipment

Aggregation plates, equilibrated (see Preparing the Aggregation Plate)

Incubator, humidified, at 37°C, 5% CO2

. . . [Full Text of this Article]


METHOD


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This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
A. Nagy, M. Gertsenstein, K. Vintersten, and R. Behringer
Analyzing Glucose Phosphate Isomerase Isozymes in Chimeric Mouse Tissues by Electrophoresis
Cold Spring Harb Protoc, September 1, 2008; 2008(10): pdb.prot4813 - pdb.prot4813.
[Abstract] [Full Text]