Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4424

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Assembling Aggregates between Embryonic Stem (ES) Cells and Diploid Embryos

Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer

This protocol was adapted from "Production of Chimeras," Chapter 11, in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes a method for assembling aggregates between ES cells and diploid embryos. The resulting chimeras are useful for separating certain extraembryonic phenotypes from phenotypes in the embryo proper, since the diploid embryo contributes to all parts of the conceptus, but the ES cell component does not contribute to the trophoblast or yolk sac endoderm.


MATERIALS

Reagents

Embryos, 2.5-days post coitum (dpc) (see Collecting Two-Cell- to Compacted Morula-Stage Embryos)

ES cells (see Preparing Embryonic Stem (ES) Cells for Aggregation)

Gelatin, 0.1% solution (e.g., Sigma) in H2O, . . . [Full Text of this Article]

Equipment


METHOD


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This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
A. Nagy, M. Gertsenstein, K. Vintersten, and R. Behringer
Analyzing Glucose Phosphate Isomerase Isozymes in Chimeric Mouse Tissues by Electrophoresis
Cold Spring Harb Protoc, September 1, 2008; 2008(10): pdb.prot4813 - pdb.prot4813.
[Abstract] [Full Text]