Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.ip19

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Live Imaging of Caenorhabditis elegans: Examples

Benjamin Podbilewicz and Yosef Gruenbaum

Adapted from "Live Imaging of Caenorhabditis elegans," Chapter 20, in Live Cell Imaging: A Laboratory Manual (eds. Goldman and Spector). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 100 words of the full text of this article appear below.


First Divisions in Early Embryos and Chromatin Dynamics in Lamin Mutants

To image early cleavages and chromatin dynamics, it is convenient to use histone H2B fused to GFP or lamin::GFP. Time-lapse movies can be obtained using conventional confocal microscope systems and their included software. Early embryos dissected from transgenic hermaphrodites are placed with egg salts on agar pads (e.g., Live Imaging of Caenorhabditis elegans: Preparation of Samples). Chromatin dynamics can be followed easily, and wild-type embryonic cells can be compared with mutants or RNAi-treated embryos [for normal lamin staining, see Fig. 1 and for chromosomal defects in lamin(RNAi) embryos, see Movie 1 ].

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  		Figure 1. A stereo pair showing three-dimensional views . . . [Full Text of this Article]

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Simultaneous Nomarski and GFP Imaging of Elongating Epidermal Cells in Embryos

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Analysis of Individual Larvae or Adults Using Confocal Microscopy and Three-Dimensional Reconstructions of Vulval Rings

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Live Imaging of Caenorhabditis elegans: Observation of Nematodes and Data Collection
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Cold Spring Harb Protoc 2006: 18. [Extract] [Full Text]

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Live Imaging of Caenorhabditis elegans: Preparation of Samples
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