Topic Introduction

Assays with Protein Arrays

Adapted from "Characterization of Protein Complexes," Chapter 10, in Proteins and Proteomics (ed. Simpson). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

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Protein-Protein Interactions

Protein arrays can be used to screen for protein-protein interactions. For this application, a protein microarray is incubated with a fluorescently labeled protein, the array is washed, and stable interactions are identified by scanning the slide for fluorescent spots (see Fig. 1 ). It is also possible to probe the array with an epitope-, hapten-, or biotin-tagged protein and to subsequently visualize bound proteins with a fluorescently labeled antibody or streptavidin conjugate. However, direct labeling of the protein of interest produces the best results. For a labeling and probing protocol for protein-protein interactions, see Protein Arrays: Labeling the Protein and . . . [Full Text of this Article]

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Protein-Small Molecule Interactions

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Kinase-Substrate Interactions

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Protein Profiling

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Reading and Interpreting the Arrays