Recipe

Polybrene

(10 mg/ml) Dissolve Polybrene (Aldrich) at a concentration of 10 mg/ml in H2O and sterilize the solution by passing it through a 0.22-μm filter. Store the solution as small aliquots (0.25-ml) at -20°C until needed. Discard aliquots after use.

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  1. doi:10.1101/pdb.rec8792 Cold Spring Harb Protoc 2006: pdb.rec8792-

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