Recipe

IEF buffer

9 M urea

324 mM dithiothreitol (DTT)

2% pharmalytes 3-10 (Amersham Biosciences)

0.5% Triton X-100

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  1. doi:10.1101/pdb.rec10590 Cold Spring Harb Protoc 2006: pdb.rec10590-
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