King’s B medium
10 g proteose peptone #2 (DIFCO)
1.5 g anhydrous K2HPO4
15 g glycerol
5 mL MgSO4 (1 M; sterile)
Antibiotics (as needed)
Add H2O to first three ingredients to bring volume to 1 L. Adjust the pH to 7.0 with HCl. Autoclave and then add 5 mL of sterile 1 M MgSO4. If MgSO4 is added before autoclaving, the medium becomes cloudy.
Add antibiotics (as needed).
