Recipe

Tissue lysis buffer

Reagent Quantity (for 20 mL) Final concentration
Tris-Cl (1 M, pH 8.0-8.5) 2.0 mL 100 mM
NaCl (5 M) 0.8 mL 200 mM
SDS (10% [w/v] in sterile H2O) 0.4 mL 0.2% (w/v)
EDTA (0.5 M) 0.2 mL 5 mM
Proteinase K (recombinant, PCR-grade) 2.0 mg 100 μg/mL
H2O 16.6 mL
Store at 4°C for up to 12 mo.
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  1. doi:10.1101/pdb.rec11948 Cold Spring Harb Protoc 2009: pdb.rec11948-
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