Recipe

Detergent solution

Reagent Quantity (for 50 mL) Final concentration
EDTA (0.5 M, pH 8.0) 100 μL 1 mM
NaCl (1 M) 7.5 mL 150 mM
SDS (sodium dodecyl sulfate; 10%) 5 mL 1.0%
Tris-Cl (0.5 M, pH 7.5) 5 mL 50 mM
Tween 20 250 μL 0.5%
H2O to 50 mL
Store at room temperature.
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  1. doi:10.1101/pdb.rec12331 Cold Spring Harb Protoc 2010: pdb.rec12331-
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