Recipe

Imaging buffer

Reagent Amount to add (for 10 mL) Final concentration
HEPES buffer 8.6 mL
D-Glucose (5% stock in HEPES buffer) 0.4 mL 0.2%
Fetal bovine serum 1 mL 10%
Imaging buffer can be stored at −20°C for up to 7 d.
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  1. doi:10.1101/pdb.rec12266 Cold Spring Harb Protoc 2010: pdb.rec12266-
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