Miller LB agar plates
MATERIALS
Reagents
Antibiotic of choice
Ampicillin sodium sulfate (50 mg/mL; Sigma-Aldrich)
Kanamycin monosulfate (30 mg/mL; Fisher Scientific)
Filter-sterilize and store antibiotics at -20°C.
LB agar, Miller (Sigma-Aldrich)
Equipment
Aluminum foil
Autoclave
Flask, 2-L
Petri dishes, 100- × 10-mm
Plastic bags
Tape
Thermometer
METHOD
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1. Dissolve 40 g of Miller LB agar in 1 L of H2O in a 2-L flask. Cover with foil and autoclave.
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2. Monitor the temperature as the solution cools. When the temperature reaches ~ 50°C, add 1 mL of the kanamycin or ampicillin stock solution.
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3. Pour ~10 mL into each Petri dish. Swirl to coat the plates. Allow the agar to solidify at room temperature.
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4. Place plates into a plastic bag. Seal with tape. Store at 4°C.
