Recipe

M9 minimal medium (standard)

Reagent Amount to add (for 100 mL)
M9 salts (5X) 20 mL
Glucose (20%; Sigma-Aldrich)a 2 mL
MgSO4 (1 M; Fisher Scientific)b 200 μL
CaCl2 (1 M; Fisher Scientific)b 10 μL
H2O 78 mL
aFilter-sterilize and store at 4°C.
bAutoclave and store at room temperature.
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  1. doi:10.1101/pdb.rec12295 Cold Spring Harb Protoc 2010: pdb.rec12295-
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