Serum-free medium for kidney culture
DMEM/F-12 medium containing 15 mM HEPES (Invitrogen/GIBCO 11330)
10 mM HEPES (Sigma H4034)
1.1 mg/mL Sodium bicarbonate (Sigma S6297)
10 nM Sodium selenite (Na2SeO3•5H2O) (Sigma S5261)
10-11 M Prostaglandin E1 (Sigma P7527)
5 μg/mL Iron-saturated transferrin (Sigma T0665)
Filter-sterilize the medium after mixing all ingredients.
Most DMEM/F-12 formulations contain 15 mM HEPES. Supplemental HEPES is added to obtain a total final concentration of 25 mM HEPES in the serum-free medium.
