aCSF for Hippocampus
| Reagent | Amount to add (for 2 L) | Final concentration |
|---|---|---|
| NaCl | 14.84 g | 127 mm |
| NaHCO3 | 4.2 g | 25 mm |
| NaH2PO4•H2O | 0.35 g | 1.25 mm |
| KCl | 0.37 g | 2.5 mm |
| d-glucose | 9.01 g | 25 mm |
| 1 m MgCl2 | 2 mL | 1 mm |
| 1 m CaCl2 | 4 mL | 2 mm |
| H2O | to 2 L | – |
Bubble with carbogen (95% O2/5% CO2) for 15–20 min. Add the CaCl2 only after bubbling with carbogen. Adjust the pH to 7.25–7.35 with NaOH. Check the osmolarity; it should be ∼310–320 mOsm. Store at 4°C.
- © 2012 Cold Spring Harbor Laboratory Press
