Recipe

In Vitro Calibration Solutions

0–40 mm KCl

10 mm HEPES buffer

10 mm Na+-gluconate

Adjust osmolarity to 310 mOsmol/L with K+-gluconate. Adjust pH to 7.4 with KOH.

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  1. doi:10.1101/pdb.rec070698 Cold Spring Harb Protoc 2012: pdb.rec070698- © 2012 Cold Spring Harbor Laboratory Press

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