Gel Elution Buffer
| Reagent | Quantity (for 500 mL) |
Final concentration |
|---|---|---|
| Tris–HCl (1 m, pH 7.5) | 10 mL | 20 mm |
| Sodium acetate (2.5 m, prepared without pH adjustment) | 50 mL | 0.25 m |
| EDTA (0.5 m, pH 8.0) | 1 mL | 1 mm |
| SDS (10%, w/v) | 12.5 mL | 0.25% |
| H2O | 426.5 mL | |
| If the SDS precipitates, warm to 37°C until the precipitate disappears. Store indefinitely at room temperature. | ||
- © 2013 Cold Spring Harbor Laboratory Press
