Whole-cell 3D STORM imaging of the mitochondrial network in a BS-C-1 cell. (A) A 3D STORM image of the mitochondrial outer membrane. Axial stepping of the objective was used to obtain an imaging depth of 3 µm. The z-dimension position is encoded according to the inset color bar. (B) Magnified vertical cross sections of several mitochondria from the image in A. Localizations from each z slice are uniquely colored by the slice in which they were originally recorded with the color index bar shown on the right. The arrows indicate regions of overlap between adjacent z slices of the mitochondrion in which the alignment error in z is estimated to be ∼18 nm after correction for spherical aberration. (Adapted from Huang et al. 2008a.)