| Cesium glutamate |
120 mm |
| EGTA (or tetracesium BAPTA) |
10 mm |
| HEPES |
10 mm |
| MgCl2 |
3 mm |
| NaCl |
8 mm |
| IP3 |
0.05 mm |
|
|
| Adjust pH to 7.2 with CsOH (not below 7.2 because CRAC channels are blocked by low pH, a pH of up to 7.4 can also be used).
Adjust osmolarity to ∼300 mOsm. Sterile-filter, then aliquot and store at −20°C. Add IP3 to the pipette solution before recording. Use this solution within 3 mo. Note that some buffering agents (e.g., BAPTA) are
unstable at room temperature and one should follow instructions of the supplier for storage and handling.
|
