Culture Medium for Retroviral Vector Production
| Dulbecco’s modified Eagle’s medium (Invitrogen 41965-039) | 500 mL |
| Fetal bovine serum (Invitrogen 16000-044) | 50 mL |
| l-glutamine, 200 mm (Invitrogen 25030-024) | 5 mL |
| Sodium pyruvate, 100 mm (Invitrogen 11360-039) | 5 mL |
| Nonessential amino acids, 10 mm (Invitrogen 11140-035) | 5 mL |
| Add 5 mL penicillin–streptomycin (Invitrogen 15070-063) as needed. Store at 4°C. | |
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