| Yeast extract |
24 g |
24 g/L |
| Tryptone |
20 g |
20 g/L |
| Glycerol |
4 mL |
4 mL/L |
| Phosphate buffer (0.17 m KH2PO4, 0.72 m K2HPO4)
|
100 mL |
0.017 m KH2PO4, 0.072 m K2HPO4 |
| Add 900 mL of deionized water to 24 g of yeast extract, 20 g of tryptone, and 4 mL of glycerol. Shake or stir until the solutes
have dissolved and sterilize by autoclaving for 20 min at 15 psi (1.05 kg/cm2). Allow the solution to cool to ∼60°C and add 100 mL of sterile phosphate buffer. Store TB at room temperature; it will keep
for at least 1 yr.
|
