Single-Channel Recording of Glycine Receptors in Human Embryonic Kidney (HEK) Cells
- Leibniz-Institut für Molekulare Pharmakologie (FMP), 13125 Berlin, Germany; NeuroCure, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany
Abstract
This protocol describes how to record the single-channel activity of recombinant homomeric glycine receptors expressed in human embryonic kidney (HEK) cells. Cell-attached recordings readily reveal the large conductance (90 pS) and distinctive clusters of activations at high glycine concentration. This method for obtaining equilibrium recordings can be adapted to any ion channel receptor. The necessary extensions to outside-out patch for nonequilibrium recordings are also described, as are basic analyses of channel properties and activity.
Footnotes
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↵1 Correspondence: Plested{at}fmp-berlin.de; baranovic{at}fmp-berlin.de
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From the Ion Channels collection, edited by Paul J. Kammermeier, Ian Duguid, and Stephan Brenowitz.
- © 2016 Cold Spring Harbor Laboratory Press
