CSH Protocols, Free Featured Protocols

Current Issue

As samples of our content, CSH Protocols is pleased to offer the following protocols for free access:

[Assay for Human Rad51-Mediated DNA Displacement Loop Formation]

[In Vitro Sumoylation of Recombinant Proteins and Subsequent Purification for Use in Enzymatic Assays]

Subject Categories

Antibodies
Bioinformatics/Genomics
Cell Biology
Developmental Biology
DNA Delivery/Gene Transfer
Genetics
High-Throughput Analysis
Imaging/Microscopy
Immunology
Laboratory Organisms
Molecular Biology
Neuroscience
Plant Biology
Polymerase Chain Reaction (PCR)
Proteins and Proteomics
RNA Interference (RNAi)/siRNA
Stem Cells
Transgenic Technology

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Antibodies

[Chromatin Immunoprecipitation (ChIP) of Protein Complexes: Mapping of Genomic Targets of Nuclear Proteins in Cultured Cells]

[Chromatin Immunopreciptation (ChIP) on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications]

[Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content]

[DNA Immunoprecipitation (DIP) for the Determination of DNA-Binding Specificity]

[Mapping Protein Distributions on Polytene Chromosomes by Immunostaining]

Bioinformatics / Genomics

[Assays with Protein Arrays]

[Browsing HapMap Data Using the Genome Browser]

[Choosing a Method for Phylogenetic Prediction]

[Copy Number Variation Detection via High-Density SNP Genotyping]

[Genotyping by Oligonucleotide Ligation Assay (OLA)]

[Methylated CpG Island Amplification and Microarray (MCAM) for High-Throughput Analysis of DNA Methylation]

[SNP Mining from Maize 454 EST Sequences]

[Using the Basic Local Alignment Search Tool (BLAST)]

Cell Biology

[A Sealed Preparation for Long-Term Observations of Cultured Cells]

[Bone Marrow-Derived Macrophages (BMM): Isolation and Applications]

[Chromatin Immunoprecipitation (ChIP) of Protein Complexes: Mapping of Genomic Targets of Nuclear Proteins in Cultured Cells]

[Chromatin Immunopreciptation (ChIP) on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications]

[Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content]

[Cultures of Cerebellar Granule Neurons]

[Culturing Human Embryonic Stem Cells in Feeder-Free Conditions]

[Cutaneous Two-Stage Chemical Carcinogenesis]

[Design and Cloning of an shRNA into a Lentiviral Silencing Vector: Version A]

[Development of Mammalian Cell Lines with lac Operator-Tagged Chromosomes]

[Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos]

[Fate-Mapping Technique: Using Carbocyanine Dyes for Vital Labeling of Cells in Gastrula-Stage Mouse Embryos Cultured in Vitro]

[Fetal Thymus Organ Culture]

[Immunohistochemistry of Whole-Mount Mouse Embryos]

[In Vitro Assays for the Extracellular Matrix Protein-Regulated Extravasation Process]

[Isolation and Whole-Cell Patch Clamping of Arabidopsis Guard Cell Protoplasts]

[Isolation, Culture, and Differentiaton of Progenitor Cells from the Central Nervous System]

[Live-Cell Imaging of GFP in Plants]

[Live Imaging of Caenorhabditis elegans: Examples]

[Microinjection of Protein Samples]

[Noninvasive Human Nuclear Transfer with Embryonic Stem Cells]

[Photoactivation-Based Labeling and In Vivo Tracking of RNA Molecules in the Nucleus]

[Preparation of Complex DNA Probe Sets for 3D FISH with up to Six Different Fluorochromes]

[Preparation of Rodent Hippocampal Slice Cultures]

[Preparing Recombinant Gonad Organ Cultures]

[Production of Endoribonuclease-Prepared Short Interfering RNAs (esiRNAs) for Specific and Effective Gene Silencing in Mammalian Cells]

[Selection of Appropriate Imaging Equipment and Methodology for Live Cell Imaging in Drosophila]

[Staining Whole Mouse Embryos for ß-Galactosidase (lacZ) Activity]

[Studying Mitosis in Cultured Mammalian Cells]

[Transcript In Situ Hybridization of Whole-Mount Embryos for Phenotype Analysis of RNAi-Treated Drosophila]

[Whole-Mount In Situ Hybridization of RNA Probes to Plant Tissues]

[Xenopus laevis Keller Explants]

Developmental Biology

[Culturing Human Embryonic Stem Cells in Feeder-Free Conditions]

[Design and Cloning of an shRNA into a Lentiviral Silencing Vector: Version A]

[Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos]

[Fate-Mapping Technique: Using Carbocyanine Dyes for Vital Labeling of Cells in Gastrula-Stage Mouse Embryos Cultured in Vitro]

[Fetal Thymus Organ Culture]

[Immunohistochemistry of Whole-Mount Mouse Embryos]

[Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi)]

[Isolation, Culture, and Differentiaton of Progenitor Cells from the Central Nervous System]

[Microinjection of dsRNA into Mouse Oocytes and Early Embryos]

[Mouse Mutagenesis Using N-Ethyl-N-Nitrosourea (ENU)]

[Noninvasive Human Nuclear Transfer with Embryonic Stem Cells]

[Planarians: A Versatile and Powerful Model System for Molecular Studies of Regeneration, Adult Stem Cell Regulation, Aging, and Behavior]

[Preparing Recombinant Gonad Organ Cultures]

[Production of Endoribonuclease-Prepared Short Interfering RNAs (esiRNAs) for Specific and Effective Gene Silencing in Mammalian Cells]

[Staining Whole Mouse Embryos for ß-Galactosidase (lacZ) Activity]

[The Genus Antirrhinum (Snapdragon): A Flowering Plant Model for Evolution and Development]

[Transcript In Situ Hybridization of Whole-Mount Embryos for Phenotype Analysis of RNAi-Treated Drosophila]

[Xenopus laevis Keller Explants]

DNA Delivery/Gene Transfer

[Design and Cloning of an shRNA into a Lentiviral Silencing Vector: Version A]

[Development of Mammalian Cell Lines with lac Operator-Tagged Chromosomes]

[Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos]

[Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi)]

[In Planta Transformation of Arabidopsis]

[Microinjection of dsRNA into Mouse Oocytes and Early Embryos]

Genetics

[Browsing HapMap Data Using the Genome Browser]

[Choosing a Method for Phylogenetic Prediction]

[Copy Number Variation Detection via High-Density SNP Genotyping]

[Genotyping by Oligonucleotide Ligation Assay (OLA)]

[Karyotyping Mouse Cells]

[Mapping Protein Distributions on Polytene Chromosomes by Immunostaining]

[Methylated CpG Island Amplification and Microarray (MCAM) for High-Throughput Analysis of DNA Methylation]

[Mouse Mutagenesis Using N-Ethyl-N-Nitrosourea (ENU)]

[Production of Endoribonuclease-Prepared Short Interfering RNAs (esiRNAs) for Specific and Effective Gene Silencing in Mammalian Cells]

[SNP Mining from Maize 454 EST Sequences]

[Using the Basic Local Alignment Search Tool (BLAST)]

High-Throughput Analysis

[Analysis of Protein Interactions with Immobilized Peptide Arrays Synthesized on Membrane Supports]

[Assays with Protein Arrays]

[Copy Number Variation Detection via High-Density SNP Genotyping]

[Gateway-Compatible Yeast One-Hybrid Screens]

[In Vitro Screening for Regulated Transcription Factors with Differential Display of DNA-Binding Proteins (DDDP)]

[Methylated CpG Island Amplification and Microarray (MCAM) for High-Throughput Analysis of DNA Methylation]

[Mouse Mutagenesis Using N-Ethyl-N-Nitrosourea (ENU)]

[Production of Endoribonuclease-Prepared Short Interfering RNAs (esiRNAs) for Specific and Effective Gene Silencing in Mammalian Cells]

Imaging / Microscopy

[A Sealed Preparation for Long-Term Observations of Cultured Cells]

[Development of Mammalian Cell Lines with lac Operator-Tagged Chromosomes]

[Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos]

[Fate-Mapping Technique: Using Carbocyanine Dyes for Vital Labeling of Cells in Gastrula-Stage Mouse Embryos Cultured in Vitro]

[Live-Cell Imaging of GFP in Plants]

[Live Imaging of Caenorhabditis elegans: Examples]

[Microinjection of Protein Samples]

[Photoactivation-Based Labeling and In Vivo Tracking of RNA Molecules in the Nucleus]

[Preparation of Complex DNA Probe Sets for 3D FISH with up to Six Different Fluorochromes]

[Preparation of Rodent Hippocampal Slice Cultures]

[Selection of Appropriate Imaging Equipment and Methodology for Live Cell Imaging in Drosophila]

[Studying Mitosis in Cultured Mammalian Cells]

Immunology

[Bone Marrow-Derived Macrophages (BMM): Isolation and Applications]

[Cutaneous Two-Stage Chemical Carcinogenesis]

[Fetal Thymus Organ Culture]

Laboratory Organisms

[Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos]

[Fate-Mapping Technique: Using Carbocyanine Dyes for Vital Labeling of Cells in Gastrula-Stage Mouse Embryos Cultured in Vitro]

[Immunohistochemistry of Whole-Mount Mouse Embryos]

[Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi)]

[In Planta Transformation of Arabidopsis]

[Isolation and Whole-Cell Patch Clamping of Arabidopsis Guard Cell Protoplasts]

[Karyotyping Mouse Cells]

[Live-Cell Imaging of GFP in Plants]

[Live Imaging of Caenorhabditis elegans: Examples]

[Maintenance of a Drosophila Laboratory: General Procedures]

[Mapping Protein Distributions on Polytene Chromosomes by Immunostaining]

[Measurement of Courtship Behavior in Drosophila melanogaster]

[Mouse Mutagenesis Using N-Ethyl-N-Nitrosourea (ENU)]

[Planarians: A Versatile and Powerful Model System for Molecular Studies of Regeneration, Adult Stem Cell Regulation, Aging, and Behavior]

[Preparation of Rodent Hippocampal Slice Cultures]

[Selection of Appropriate Imaging Equipment and Methodology for Live Cell Imaging in Drosophila]

[Staining Whole Mouse Embryos for ß-Galactosidase (lacZ) Activity]

[The Genus Antirrhinum (Snapdragon): A Flowering Plant Model for Evolution and Development]

[Transcript In Situ Hybridization of Whole-Mount Embryos for Phenotype Analysis of RNAi-Treated Drosophila]

[Whole-Mount In Situ Hybridization of RNA Probes to Plant Tissues]

[Xenopus laevis Keller Explants]

Molecular Biology

[Analysis of Protein Interactions with Immobilized Peptide Arrays Synthesized on Membrane Supports]

[Chromatin Immunoprecipitation (ChIP) of Protein Complexes: Mapping of Genomic Targets of Nuclear Proteins in Cultured Cells]

[Chromatin Immunopreciptation (ChIP) on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications]

[Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content]

[DNA Immunoprecipitation (DIP) for the Determination of DNA-Binding Specificity]

[Cutaneous Two-Stage Chemical Carcinogenesis]

[Design and Cloning of an shRNA into a Lentiviral Silencing Vector: Version A]

[Development of Mammalian Cell Lines with lac Operator-Tagged Chromosomes]

[Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos]

[Gateway-Compatible Yeast One-Hybrid Screens]

[Genotyping by Oligonucleotide Ligation Assay (OLA)]

[Immunohistochemistry of Whole-Mount Mouse Embryos]

[In Vitro Screening for Regulated Transcription Factors with Differential Display of DNA-Binding Proteins (DDDP)]

[Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi)]

[Mapping Protein Distributions on Polytene Chromosomes by Immunostaining]

[Methylated CpG Island Amplification and Microarray (MCAM) for High-Throughput Analysis of DNA Methylation]

[Microinjection of dsRNA into Mouse Oocytes and Early Embryos]

[Mouse Mutagenesis Using N-Ethyl-N-Nitrosourea (ENU)]

[Photoactivation-Based Labeling and In Vivo Tracking of RNA Molecules in the Nucleus]

[Preparation of Complex DNA Probe Sets for 3D FISH with up to Six Different Fluorochromes]

[Production of Endoribonuclease-Prepared Short Interfering RNAs (esiRNAs) for Specific and Effective Gene Silencing in Mammalian Cells]

[Preparation of GST Fusion Proteins]

[Quantitation of DNA and RNA]

[SNP Mining from Maize 454 EST Sequences]

[Staining Whole Mouse Embryos for ß-Galactosidase (lacZ) Activity]

[Transcript In Situ Hybridization of Whole-Mount Embryos for Phenotype Analysis of RNAi-Treated Drosophila]

[Whole-Mount In Situ Hybridization of RNA Probes to Plant Tissues]

Neuroscience

[Cultures of Cerebellar Granule Neurons]

[Measurement of Courtship Behavior in Drosophila melanogaster]

[Preparation of Rodent Hippocampal Slice Cultures]

Plant Biology

[In Planta Transformation of Arabidopsis]

[Isolation and Whole-Cell Patch Clamping of Arabidopsis Guard Cell Protoplasts]

[Isolation, Culture, and Differentiaton of Progenitor Cells from the Central Nervous System]

[Live-Cell Imaging of GFP in Plants]

[Whole-Mount In Situ Hybridization of RNA Probes to Plant Tissues]

[SNP Mining from Maize 454 EST Sequences]

[The Genus Antirrhinum (Snapdragon): A Flowering Plant Model for Evolution and Development]

Polymerase Chain Reaction (PCR)

[Genotyping by Oligonucleotide Ligation Assay (OLA)]

[Methylated CpG Island Amplification and Microarray (MCAM) for High-Throughput Analysis of DNA Methylation]

[Preparation of Complex DNA Probe Sets for 3D FISH with up to Six Different Fluorochromes]

Proteins and Proteomics

[Analysis of Complex Protein Mixtures Using Multidimensional Protein Identification Technology (MuDPIT)]

[Analysis of Protein Interactions with Immobilized Peptide Arrays Synthesized on Membrane Supports]

[Assays with Protein Arrays]

[Chromatin Immunoprecipitation (ChIP) of Protein Complexes: Mapping of Genomic Targets of Nuclear Proteins in Cultured Cells]

[Chromatin Immunopreciptation (ChIP) on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications]

[Development of Mammalian Cell Lines with lac Operator-Tagged Chromosomes]

[Gateway-Compatible Yeast One-Hybrid Screens]

[HPLC Separation of Digested Proteins and Preparation for Matrix-Assisted Laser Desorption/Ionization Analysis]

[In Vitro Assays for the Extracellular Matrix Protein-Regulated Extravasation Process]

[In Vitro Screening for Regulated Transcription Factors with Differential Display of DNA-Binding Proteins (DDDP)]

[In Vivo Isotopic Labeling of Proteins for Quantitative Proteomics]

[Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi)]

[Mapping Protein Distributions on Polytene Chromosomes by Immunostaining]

[Microinjection of Protein Samples]

[Preparation of GST Fusion Proteins]

RNA Interference (RNAi)/siRNA

[Design and Cloning of an shRNA into a Lentiviral Silencing Vector: Version A]

[Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi)]

[Microinjection of dsRNA into Mouse Oocytes and Early Embryos]

[Production of Endoribonuclease-Prepared Short Interfering RNAs (esiRNAs) for Specific and Effective Gene Silencing in Mammalian Cells]

[Transcript In Situ Hybridization of Whole-Mount Embryos for Phenotype Analysis of RNAi-Treated Drosophila]

Stem Cells

[Culturing Human Embryonic Stem Cells in Feeder-Free Conditions]

[Isolation, Culture, and Differentiaton of Progenitor Cells from the Central Nervous System]

[Noninvasive Human Nuclear Transfer with Embryonic Stem Cells]

Transgenic Technology

[Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos]

[Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi)]

[In Planta Transformation of Arabidopsis]

[Microinjection of dsRNA into Mouse Oocytes and Early Embryos]

[Staining Whole Mouse Embryos for ß-Galactosidase (lacZ) Activity]

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