Table of Contents

Protocols

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  RNAi in the Red Flour Beetle (Tribolium)

  • Nico Posnien,
  • Johannes Schinko,
  • Daniela Grossmann,
  • Teresa D. Shippy,
  • Barbora Konopova,
  • and Gregor Bucher

  Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5256
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  Concurrent In Situ Hybridization and Antibody Staining in Red Flour Beetle (Tribolium) Embryos

  • Teresa D. Shippy,
  • Cassondra M. Coleman,
  • Yoshinori Tomoyasu,
  • and Susan J. Brown

  Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5257
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  Single and Double Whole-Mount In Situ Hybridization in Red Flour Beetle (Tribolium) Embryos

  • Johannes Schinko,
  • Nico Posnien,
  • Sebastian Kittelmann,
  • Nikolaus Koniszewski,
  • and Gregor Bucher

  Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5258
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  Red Flour Beetle (Tribolium) Germline Transformation and Insertional Mutagenesis

  • Andreas J. Berghammer,
  • Markus Weber,
  • Jochen Trauner,
  • and Martin Klingler

  Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5259
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  Analyzing Microarray Data Using WebArray

  • Yipeng Wang,
  • Michael McClelland,
  • and Xiao-Qin Xia

  Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5260
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  Dissection and Fixation of Large Milkweed Bug (Oncopeltus) Embryos

  • Paul Liu and
  • Thomas C. Kaufman

  Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5261
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  In Situ Hybridization of Large Milkweed Bug (Oncopeltus) Tissues

  • Paul Liu and
  • Thomas C. Kaufman

  Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5262
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  Axolotl (Ambystoma mexicanum) In Vitro Fertilization

  • Shahryar Khattak and
  • Elly M. Tanaka

  Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5263
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  Generation of Transgenic Axolotls (Ambystoma mexicanum)

  • Shahryar Khattak,
  • Tobias Richter,
  • and Elly M. Tanaka

  Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5264
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  Axolotl (Ambystoma mexicanum) Embryonic Transplantation Methods

  • Eugen Nacu,
  • Dunja Knapp,
  • Elly M. Tanaka,
  • and Hans H. Epperlein

  Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5265
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  Grafting Axolotl (Ambystoma mexicanum) Limb Skin and Cartilage from GFP+ Donors to Normal Hosts

  • Martin Kragl and
  • Elly M. Tanaka

  Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5266
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  Axolotl (Ambystoma mexicanum) Limb and Tail Amputation

  • Martin Kragl and
  • Elly M. Tanaka

  Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5267
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  Induction of Metamorphosis in Axolotls (Ambystoma mexicanum)

  • Robert B. Page and
  • S. Randal Voss

  Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5268
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  Chromosome Orientation Fluorescence In Situ Hybridization (CO-FISH)

  • Eli S. Williams and
  • Susan M. Bailey

  Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5269
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  Use of the Sleeping Beauty Transposon System for Stable Gene Expression in Mouse Embryonic Stem Cells

  • Ann E. Davidson,
  • Theresa E. Gratsch,
  • Maria H. Morell,
  • K. Sue O’Shea,
  • and Catherine E. Krull

  Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5270
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  Isolation and Cloning of High-Molecular-Weight Metagenomic DNA from Soil Microorganisms

  • Mark R. Liles,
  • Lynn L. Williamson,
  • Jitsupang Rodbumrer,
  • Vigdis Torsvik,
  • Larissa C. Parsley,
  • Robert M. Goodman,
  • and Jo Handelsman

  Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5271
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  Preparation of Coverslips for Neuronal Cultures

  • Cristóvão Albuquerque,
  • Donald J. Joseph,
  • Papiya Choudhury,
  • and Amy B. MacDermott

  Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5272
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  Dissection, Plating, and Maintenance of Cortical Astrocyte Cultures

  • Cristóvão Albuquerque,
  • Donald J. Joseph,
  • Papiya Choudhury,
  • and Amy B. MacDermott

  Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5273
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  Dissection, Plating, and Maintenance of Dorsal Horn Neuron Cultures

  • Cristóvão Albuquerque,
  • Donald J. Joseph,
  • Papiya Choudhury,
  • and Amy B. MacDermott

  Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5274
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  Dissection, Plating, and Maintenance of Dorsal Root Ganglion Neurons for Monoculture and for Coculture with Dorsal Horn Neurons

  • Cristóvão Albuquerque,
  • Donald J. Joseph,
  • Papiya Choudhury,
  • and Amy B. MacDermott

  Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5275
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  Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors

  • Els Verhoeyen and
  • François-Loïc Cosset

  Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5276
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