Recipe

Array tomography fixative

Reagent Amount to add (for 4 mL) Final concentration
Paraformaldehyde, EM grade (8%; Electron Microscopy Sciences 157-8) 2 mL 4%
Phosphate-buffered saline (0.02 M, pH 7.4; Sigma-Aldrich P3813) 2 mL 0.01 M
Sucrose 0.1 g 2.5%
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  1. doi:10.1101/pdb.rec12345 Cold Spring Harb Protoc 2010: pdb.rec12345-
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