Array tomography wash buffer
| Reagent | Amount to add (for 50 mL) | Final concentration |
|---|---|---|
| Glycine | 187.5 mg | 50 mM |
| Phosphate-buffered saline (0.02 M, pH 7.4; Sigma-Aldrich P3813) | 25 mL | 0.01 M |
| Sucrose | 1.75 g | 3.5% |
| H2O | 25 mL | |
| This buffer can be prepared in advance. Store for up to 1 mo at 4°C. Discard if cloudy. | ||
