Recipe

MES running buffer

50 mM MES (2-[N-morpholino]ethanesulfonic acid)

50 mM Tris base

1 mM EDTA

0.1% (w/v) SDS

Measure the pH of this solution; it should be 7.3.

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  1. doi:10.1101/pdb.rec12358 Cold Spring Harb Protoc 2010: pdb.rec12358-
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