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Purification of Pericytes from Rodent Optic Nerve by Immunopanning

Lu Zhou,
Fabien Sohet,
and Richard Daneman

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot074955

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High-Speed Two-Photon Calcium Imaging of Neuronal Population Activity Using Acousto-Optic Deflectors

Benjamin F. Grewe and
Fritjof Helmchen

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot081778

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Measuring Endogenous I_CRAC and ORAI Currents with the Patch-Clamp Technique

Dalia Alansary,
Tatiana Kilch,
Christian Holzmann,
Christine Peinelt,
Markus Hoth,
and Annette Lis

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot073254

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The Minimal Requirements to Use Calcium Imaging to Analyze I_CRAC

Dalia Alansary,
Tatiana Kilch,
Christian Holzmann,
Christine Peinelt,
Markus Hoth,
and Annette Lis

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot073262

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Monitoring Membrane Potential with Second-Harmonic Generation

Stacy A. Wilson,
Andrew Millard,
Aaron Lewis,
and Leslie M. Loew

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot081786

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Manual Hematoxylin and Eosin Staining of Mouse Tissue Sections

Robert D. Cardiff,
Claramae H. Miller,
and Robert J. Munn

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot073411

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Manual Immunohistochemistry Staining of Mouse Tissues Using the Avidin–Biotin Complex (ABC) Technique

Robert D. Cardiff,
Claramae H. Miller,
and Robert J. Munn

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot073429

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A High-Throughput, Multiplex Cell Death Assay Using an RNAi Screening Approach

Katrina J. Falkenberg,
Darren N. Saunders,
and Kaylene J. Simpson

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot080267

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RNase Footprinting to Map Sites of RNA–Protein Interactions

Timothy W. Nilsen

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot080788

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Reverse Footprinting to Map Sites of RNA–Protein Interactions

Timothy W. Nilsen

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot080804

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3′-End Labeling of RNA with Yeast Poly(A) Polymerase and 3′-Deoxyadenosine 5′-[α-³²P]Triphosphate

Donald C. Rio

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot080770

TOPIC INTRODUCTIONS

Analysis of Mouse Model Pathology: A Primer for Studying the Anatomic Pathology of Genetically Engineered Mice

Purification and Culture of Central Nervous System Pericytes

Nitrophenyl-Based Caged Neurotransmitters

High-Throughput Approaches to Measuring Cell Death

Patch-Clamp Measurement of I_CRAC and ORAI Channel Activity

PROTOCOLS

Purification of Pericytes from Rodent Optic Nerve by Immunopanning

High-Speed Two-Photon Calcium Imaging of Neuronal Population Activity Using Acousto-Optic Deflectors

Measuring Endogenous I_CRAC and ORAI Currents with the Patch-Clamp Technique

The Minimal Requirements to Use Calcium Imaging to Analyze I_CRAC

Monitoring Membrane Potential with Second-Harmonic Generation

Manual Hematoxylin and Eosin Staining of Mouse Tissue Sections

Manual Immunohistochemistry Staining of Mouse Tissues Using the Avidin–Biotin Complex (ABC) Technique

A High-Throughput, Multiplex Cell Death Assay Using an RNAi Screening Approach

RNase Footprinting to Map Sites of RNA–Protein Interactions

Reverse Footprinting to Map Sites of RNA–Protein Interactions

3′-End Labeling of RNA with Yeast Poly(A) Polymerase and 3′-Deoxyadenosine 5′-[α-³²P]Triphosphate

This Issue

Current Issue

From the cover

Table of Contents

TOPIC INTRODUCTIONS

Analysis of Mouse Model Pathology: A Primer for Studying the Anatomic Pathology of Genetically Engineered Mice

Purification and Culture of Central Nervous System Pericytes

Nitrophenyl-Based Caged Neurotransmitters

High-Throughput Approaches to Measuring Cell Death

Patch-Clamp Measurement of ICRAC and ORAI Channel Activity

PROTOCOLS

Purification of Pericytes from Rodent Optic Nerve by Immunopanning

High-Speed Two-Photon Calcium Imaging of Neuronal Population Activity Using Acousto-Optic Deflectors

Measuring Endogenous ICRAC and ORAI Currents with the Patch-Clamp Technique

The Minimal Requirements to Use Calcium Imaging to Analyze ICRAC

Monitoring Membrane Potential with Second-Harmonic Generation

Manual Hematoxylin and Eosin Staining of Mouse Tissue Sections

Manual Immunohistochemistry Staining of Mouse Tissues Using the Avidin–Biotin Complex (ABC) Technique

A High-Throughput, Multiplex Cell Death Assay Using an RNAi Screening Approach

RNase Footprinting to Map Sites of RNA–Protein Interactions

Reverse Footprinting to Map Sites of RNA–Protein Interactions

3′-End Labeling of RNA with Yeast Poly(A) Polymerase and 3′-Deoxyadenosine 5′-[α-32P]Triphosphate

This Issue

Current Issue

From the cover

Patch-Clamp Measurement of I_CRAC and ORAI Channel Activity

Measuring Endogenous I_CRAC and ORAI Currents with the Patch-Clamp Technique

The Minimal Requirements to Use Calcium Imaging to Analyze I_CRAC

3′-End Labeling of RNA with Yeast Poly(A) Polymerase and 3′-Deoxyadenosine 5′-[α-³²P]Triphosphate