Table of Contents

July 2016; 2016 (7)

TOPIC INTRODUCTIONS

  • Historical Evolution of Laboratory Strains of Saccharomyces cerevisiae

    • Edward J. Louis
    Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.top077750

  • Guide RNAs: A Glimpse at the Sequences that Drive CRISPR–Cas Systems

    • Alexandra E. Briner and
    • Rodolphe Barrangou
    Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.top090902

  • Protein Complex Purification by Affinity Capture

    • John LaCava,
    • Javier Fernandez-Martinez,
    • Zhanna Hakhverdyan,
    • and Michael P. Rout
    Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.top077545

  • Fixed-Cell Imaging of Schizosaccharomyces pombe

    • Iain M. Hagan and
    • Steven Bagley
    Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.top079830

  • Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel Currents in Neurons

    • Mala M. Shah
    Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.top087346

PROTOCOLS

  • Optimized Affinity Capture of Yeast Protein Complexes

    • John LaCava,
    • Javier Fernandez-Martinez,
    • Zhanna Hakhverdyan,
    • and Michael P. Rout
    Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot087932

  • Native Elution of Yeast Protein Complexes Obtained by Affinity Capture

    • John LaCava,
    • Javier Fernandez-Martinez,
    • and Michael P. Rout
    Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot087940

  • Density Gradient Ultracentrifugation to Isolate Endogenous Protein Complexes after Affinity Capture

    • Javier Fernandez-Martinez,
    • John LaCava,
    • and Michael P. Rout
    Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot087957

  • Prediction and Validation of Native and Engineered Cas9 Guide Sequences

    • Alexandra E. Briner,
    • Emily D. Henriksen,
    • and Rodolphe Barrangou
    Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot086785

  • Triggering Apoptosis in Hematopoietic Cells with Cytotoxic Drugs

    • Lisa C. Crowley,
    • Brooke J. Marfell,
    • Adrian P. Scott,
    • and Nigel J. Waterhouse
    Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot087130

  • Triggering Death of Adherent Cells with Ultraviolet Radiation

    • Lisa C. Crowley and
    • Nigel J. Waterhouse
    Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot087148

  • Measuring Cell Death by Trypan Blue Uptake and Light Microscopy

    • Lisa C. Crowley,
    • Brooke J. Marfell,
    • Melinda E. Christensen,
    • and Nigel J. Waterhouse
    Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot087155

  • Measuring Cell Death by Propidium Iodide Uptake and Flow Cytometry

    • Lisa C. Crowley,
    • Adrian P. Scott,
    • Brooke J. Marfell,
    • Jeanne A. Boughaba,
    • Grace Chojnowski,
    • and Nigel J. Waterhouse
    Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot087163

  • Immunofluorescence Microscopy of Schizosaccharomyces pombe Using Chemical Fixation

    • Iain M. Hagan
    Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot091017

  • Recording Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel Currents (Ih) in Neurons

    • Mala M. Shah
    Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot091462

ERRATUM

  • Erratum: Preparation of Nuclear Extracts from HeLa Cells

    • Timothy W. Nilsen
    Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.err094813

CORRIGENDUM

  • Corrigendum: Growth and the Environment of Schizosaccharomyces pombe

    • Janni Petersen and
    • Paul Russell
    Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.corr095232
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This Issue

July 2016; 2016 (7)
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  1. TOPIC INTRODUCTIONS
  2. PROTOCOLS
  3. ERRATUM
  4. CORRIGENDUM

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