Recipe

AP buffer for Aedes

Reagent Quantity (for 50 mL) Final concentration
MgCl2 (1 M) 250 μL 5 mM
NaCl (1 M) 5 mL 100 mM
Tris-Cl (1 M, pH 9.5) 5 mL 100 mM
Tween 20 50 μL 0.1%
H2O to 50 mL
Prepare just before use. Store for no more than 3 h.
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  1. doi:10.1101/pdb.rec12330 Cold Spring Harb Protoc 2010: pdb.rec12330-
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