Rich auto-induction medium

Reagent	Amount to add (for 1 L)	Final concentration
β-lactose (Sigma-Aldrich)	10 g	1%
Glucose (Sigma-Aldrich)	0.5 g	0.05%
Glycerol (100%; Fisher Scientific)^a	5 g	0.5%
KH₂PO₄ (Fisher Scientific)	6.8 g	50 mM
MgSO₄ (1 M; Fisher Scientific)^b	2 mL	2 mM
N-Z amine AS (Sigma-Aldrich)	10 g	1%
Na₂HPO₄ (Fisher Scientific)	7.1 g	50 mM
Na₂SO₄ (Fisher Scientific)	0.71 g	5 mM
NH₄Cl (Fisher Scientific)	2.67 g	50 mM
Trace metals (1000X; Sigma-Aldrich)^a	400 μL	0.2X
Yeast extract (Fisher Scientific)	5 g	0.5%

Adjust the pH to 8.0-8.2.
^aAutoclave and store at 4°C.
^bAutoclave and store at room temperature.
This medium is based on Studier’s medium ZYM-5052, with modifications for high-level protein production (Studier FW. 2005. Protein production by auto-induction in high density shaking cultures. Protein Expr Purif 41: 207-234).