Recipe

SDS sample solubilization buffer

 
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1% (w/v) SDS

100 mM Tris-HCl (pH 9.5)

To prepare 50 ml of SDS sample buffer, dissolve 0.5 g of SDS and 0.6 g of Tris base in ~40 ml of deionized H2O. Titrate to pH 9.5 with 4 N HCl, filter, and adjust the volume to 50 ml with deionized H2O.

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  1. doi:10.1101/pdb.rec10017 Cold Spring Harb Protoc 2006: pdb.rec10017-
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