Recipe

TMB substrate

 
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3, 3', 5, 5'-tetramethylbenzidine (TMB) (50 g/l stock), 50 μl

Sodium acetate (0.1 M, pH 6.6), 5 ml

Hydrogen peroxide (30%), 1 μl

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Prepare fresh before each use.

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  1. doi:10.1101/pdb.rec10070 Cold Spring Harb Protoc 2006: pdb.rec10070-
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