Recipe

3x SDS sample buffer

 
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188 mM Tris-Cl (pH 6.8)

3% SDS

30% glycerol

0.01% bromophenol blue

15% β-mercaptoethanol

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The 1.5x SDS sample buffer is diluted from the 3x stock with H2O

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  1. doi:10.1101/pdb.rec10075 Cold Spring Harb Protoc 2006: pdb.rec10075-
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