Native separation medium 3

Glycerol (25% w/w)

Hydroxypropylmethyl cellulose (HPMC) (0.2% w/w)

ProLyte 3 (14.3% w/w)

For 70 g, weigh out 10 g of ProLyte 3. Add 7.5 g of H₂O and 52.5 g of HPMC/glycerol/H₂O stock (33%/33%/33% w/w/w). Mix thoroughly by stirring. pH = 9.85 ±0.10; conductivity 302 ±12 μS/cm. Actual Consumption: ˜9 g/hr.