Recipe

CSPD assay buffer

 
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50 mM 2-amino-2-methyl-1-propanol (AMP, pH 10.3)

Filter the solution through a 0.2 μm filter, and store the solution at 4°C. AMP can be purchased from Sigma Chemical as a 1.5 M solution at pH 10.3.

1 mM MgCl2

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  1. doi:10.1101/pdb.rec10273 Cold Spring Harb Protoc 2006: pdb.rec10273-
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