Recipe

CAPS Transfer buffer

 
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10 mM CAPS (pH 10.5)

Methanol*

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*The optimal methanol concentration to use depends on the protein that will be selected by the primary antibody; 5% works well for many proteins.

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  1. doi:10.1101/pdb.rec10286 Cold Spring Harb Protoc 2006: pdb.rec10286-
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