Recipe

Denaturing buffer containing urea

 
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8 M urea

5 mM dithiothreitol

50 mM Tris-Cl (pH 7.5)

150 mM NaCl

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Warm the solution to 40ºC to dissolve the urea. Adjust the pH to 7.5 if necessary.

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  1. doi:10.1101/pdb.rec10300 Cold Spring Harb Protoc 2006: pdb.rec10300-
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