BES-buffered saline (BBS), 2X
50 mM BES (N,N-bis[2-hydroxyethyl]-2-aminoethanesulfonic acid) (EMD/Calbiochem)
1.5 mM Na2HPO4
280 mM NaCl
Mix 16.36 g of NaCl, 10.65 g of BES, and 0.21 g of Na2HPO4. Add double-distilled H2O up to 900 mL. Dissolve, titrate to pH 6.95 with 1 M NaOH, and bring volume to 1 L with H2O. Filter-sterilize and store 14-mL aliquots at 4ºC.
